Comparative Analysis of Single-Cell RNA Sequencing Methods
Christoph Ziegenhain, Beate Vieth, Swati Parekh, Björn Reinius, Amy Guillaumet-Adkins, Martha Smets, Heinrich Leonhardt, Holger Heyn, Ines Hellmann and Wolfgang Enard
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Ziegenhain et al. generated data from mouse ESCs to systematically evaluate six prominent scRNA-seq methods. They used power simulations to compare cost efficiencies, allowing for informed choice among existing protocols and providing a framework for future comparisons.
- represents the most comprehensive comparison of scRNA-seq protocols,
- provides power simulations quantifying the effect of sensitivity and precision on cost efficiency,
- offers an informed choice among six prominent scRNA-seq methods,
- provides a framework for benchmarking future protocol improvements.