Tub-tag labeling: a new approach for site-specific protein labeling
A novel chemoenzymatic approach for simple and fast site-specific protein labeling is reported in this publication. Recombinant tubulin tyrosine ligase (TTL) was repurposed to attach various unnatural tyrosine derivatives as small bioorthogonal handles to proteins containing a short tubulin-derived recognition sequence (Tub-tag). This novel strategy enables a broad range of high-yielding and fast chemoselective C-terminal protein modifications on isolated proteins or in cell lysates for applications in biochemistry, cell biology, and beyond, as demonstrated by the site-specific labeling of nanobodies, GFP, and ubiquitin.
Versatile and efficient site-specific protein functionalization by Tubulin Tyrosine Ligase.
Dominik Schumacher, Jonas Helma, Florian A. Mann, Garwin Pichler, Francesco Natale, Eberhard Krause, M.Cristina Cardoso, Christian P.R. Hackenberger, Heinrich Leonhardt